Neuropeptide B (NPB) and neuropeptide W (NPW) are members of a recently identified neuropeptide family that are ligands for two highly similar receptors, NPBW1 (GPR7) and NPBW2 (GPR8), both of which couple to Gi/o to inhibit intracellular cAMP production. Highest expression of NPBW1 mRNA and protein was identified in the amygdala and hypothalamic nuclei. Physiological studies demonstrate that intracerebroventricular infusion of NPBW1 ligands modulates feeding behaviour, nociception, and release of corticosterone, prolactin and growth hormone. NPBW1 knock out male mice have shown mild adult-onset obesity and decreased locomotor activity. They become progressively hyperglycaemic and hyperinsulinaemic.