Human KCNQ3/KCNQ5 Stable Cell Line-CHO-K1

Name: Human KCNQ3/KCNQ5 Stable Cell Line-CHO-K1
SKU: CSC-RI0044
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0044

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RI0044
Description	This cell line is engineered to overexpress human KCNQ3/KCNQ5.
Target Gene	KCNQ3/KCNQ5
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuKCNQ3/KCNQ5
Alias	KCNQ5, Kv7.5
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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In Mammals, one of the most physiologically-relevant and studied currents is the M current, named this because it is a current observed in neurons in response to addition of muscarine. The M current is inhibited by various brain peptides and M1 muscarinic acetycholine GPCRS. M currents are recorded from brain tissue are all thought to contain Kv7.3, and one combination that appears to be responsible for making M current in neurons in Kv 7.3 with Kv 7.5. These subunits are preferentially expressed in brain & sympathetic ganglia. Openers active on brain currents limit repetitive firing, and their ability to modulate overactivity in dopaminergic & serotonergic pathways is of therapeutic interest for schizophrenia, drug abuse and anxiety.

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