Gastric inhibitory polypeptide (GIP) is a 42 amino acid polypeptide synthesized by K-cells of the duodenum and small intestine. GIP’s principle physiological action is to stimulate insulin secretion from pancreatic beta cells in a glucose-dependent manner. Additional effects of GIP have been described, including effects on lipid metabolism, stimulation of glucagons and somatostatin release. GIP exerts its effects by bhinding to its specific receptor, the GIP receptor which belongs to the VIP/guucagon/secretin family of G protein-coupled receptors and is positively coupled to adenylyl cyclase but not to phospholipase C.
12 passages of continuous culture (6 weeks) at 2 passages/week