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HIV Reverse Transcriptase

For research use only. Not intended for any clinical use.
Cat.No.
EMTT4002
Description
Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Applications
Provides an excellent target for evaluating antiviral agents or inhibitors. Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.
Concentration
10-30 units/μl
Reaction Buffer
50 mM Tris HCl (pH 8.3) 6 mM MgCl 240 mM KCl 0.5 mM [3H] TTP 0.4 mM polyA (dT) 18-20 Reaction volume of 50 μl
Reaction Conditions
Incubation at 37 °C
Size/Form
2000 Units; 500 Units
Storage
20 mM potassium phosphate (pH 7.1) 1.0 mM dithiothreitol 0.02% Triton X-100 50% glycerol
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutesat 37 °C.

Publications

Q & A

Customer Reviews

Customer Q&As
How is the enzyme activity of the enzyme defined?

A: One unit of this enzyme is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid-insoluble material within 10 minutes at 37°C.

Can DNA be used as a template for amplification?

A: Yes, this enzyme can be used to amplify RNA and single-stranded DNA templates to obtain DNA strands.

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Customer Reviews
Good evaluation

Provides an excellent target for evaluating antiviral agents or inhibitors.

United States

11/21/2021

Random insertion mutations

Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.

United States

01/31/2022

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