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HIV Reverse Transcriptase

For research use only. Not intended for any clinical use.

Cat. No. :   EMTT4002

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Product Information

Cat. No. EMTT4002
Description Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Concentration 10-30 units/μl
Applications Provides an excellent target for evaluating antiviral agents or inhibitors. Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.
Size 2000 Units; 500 Units
Unit Definition One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutesat 37 °C.
Reaction Buffer 50 mM Tris HCl (pH 8.3) 6 mM MgCl 240 mM KCl 0.5 mM [3H] TTP 0.4 mM polyA (dT) 18-20 Reaction volume of 50 μl
Reaction Conditions Incubation at 37 °C
Storage 20 mM potassium phosphate (pH 7.1) 1.0 mM dithiothreitol 0.02% Triton X-100 50% glycerol
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Customer Reviews

Customer Q&As
How is the enzyme activity of the enzyme defined?

A: One unit of this enzyme is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid-insoluble material within 10 minutes at 37°C.

Can DNA be used as a template for amplification?

A: Yes, this enzyme can be used to amplify RNA and single-stranded DNA templates to obtain DNA strands.

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Customer Reviews
Good evaluation

Provides an excellent target for evaluating antiviral agents or inhibitors.

United States

Random insertion mutations

Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.

United States

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