Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Provides an excellent target for evaluating antiviral agents or inhibitors. Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.
50 mM Tris HCl (pH 8.3) 6 mM MgCl 240 mM KCl 0.5 mM [3H] TTP 0.4 mM polyA (dT) 18-20 Reaction volume of 50 μl
Incubation at 37 °C
2000 Units; 500 Units
20 mM potassium phosphate (pH 7.1) 1.0 mM dithiothreitol 0.02% Triton X-100 50% glycerol
One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutesat 37 °C.