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HIV Reverse Transcriptase

Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Provides an excellent target for evaluating antiviral agents or inhibitors. Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.
10-30 units/μl
Reaction Buffer
50 mM Tris HCl (pH 8.3) 6 mM MgCl 240 mM KCl 0.5 mM [3H] TTP 0.4 mM polyA (dT) 18-20 Reaction volume of 50 μl
Reaction Conditions
Incubation at 37 °C
2000 Units; 500 Units
20 mM potassium phosphate (pH 7.1) 1.0 mM dithiothreitol 0.02% Triton X-100 50% glycerol
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutesat 37 °C.
For research use only. Not intended for any clinical use.

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