Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMTT4002
| Cat. No. | EMTT4002 |
| Description | Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. |
| Concentration | 10-30 units/μl |
| Applications | Provides an excellent target for evaluating antiviral agents or inhibitors. Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations. |
| Size | 2000 Units; 500 Units |
| Unit Definition | One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutesat 37 °C. |
| Reaction Buffer | 50 mM Tris HCl (pH 8.3) 6 mM MgCl 240 mM KCl 0.5 mM [3H] TTP 0.4 mM polyA (dT) 18-20 Reaction volume of 50 μl |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 20 mM potassium phosphate (pH 7.1) 1.0 mM dithiothreitol 0.02% Triton X-100 50% glycerol |
A: One unit of this enzyme is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid-insoluble material within 10 minutes at 37°C.
A: Yes, this enzyme can be used to amplify RNA and single-stranded DNA templates to obtain DNA strands.
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Provides an excellent target for evaluating antiviral agents or inhibitors.
Catalyzes error-prone synthesis on DNA and RNA templates-ideal for introducing random mutations.
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