MCF-7 is a cell line that was first isolated in 1970 from the breast tissue of a 69-year old Caucasian female. Of the two mastectomies she received, the first revealed the removed tissue to be benign. Five years later, a second operation revealed malignant adenocarcinoma in a pleural effusion from which was taken cells for MCF-7. The GFP Stable Cell Line-MCF-7 constitutively expresses GFP.
Inquiry for instruction of culturing
This cryovial contains at least 1.0 × 10^6 cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The cells are tested free of microbial contamination.