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pSEX81 phagemid Vector

Cat.No.
VPT4034
Product description
pSEX81 was used for the convenient insertion of heavy and light chain variable domain coding regions and for production of functional single-chain Fv antibody-pIII fusion proteins on the surface of M13 bacteriophages. The corresponding DNA fragments of human or mouse origin can be amplified by PCR.The amplified gene fragments encoding the variable heavy or light chain domain are cloned in-frame between a signal peptide sequence of bacterial pectate lyase (pelB) for the secretion of the fusion protein into the periplasmic space, and the pIII gene of M13 bacteriophage. The VH and VL genes were joined by a DNA-fragment coding for a flexible 18 amino acid residue linker containing the first six amino acids of the CH1 constant region domain and the hydrophilic pig brain alpha-tubulin peptide sequence EEGEFSEAR. The vector backbone further provides a strong promotor, the T7 terminator, the ColE1 origin of replication, the intergenic region of phage F1 and an ampicillin resistance marker for selection.
Vector type
phagemid vector
Vector length
4,882 bp
Resistance
Amp
pSEX81 phagemid Vector
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