First introduced by the American molecular biologist, Joshua Lederberg, in the year of 1952, the term plasmid was used to describe any bacterial genetic material that exists in an extrachromosomal state. Since then, the description was narrowed to genetic elements that exists exclusively or predominantly outside of the chromosome and can replicate autonomously in order to distinguish it from viruses. Plasmids are present in archaea and eukaryotic organisms while they are often found in bacteria as circular, small, and double-stranded DNA molecules within a cell. In fact, however, plasmids commonly carry genes. Those genes can exert an influence on the survival of the organism, such as antibiotic resistance. On the other hand, plasmids are also known as replicons, which is a unit of DNA that is capable of self-replicating within a suitable host. Additionally, plasmid can be transmitted from bacterium to bacterium, even among different species.
There are mainly three mechanisms of transmission, namely transformation, transduction, as well as conjugation. Different from viruses, plasmids are “naked” DNA. They don’t encode genes necessary to encase the genetic material for transfer to a new host. Although small as they are, plasmids can conclude additional information.
Creative Biogene’s plasmid production services currently contain construct plasmid according to different requirements, robust manufacturing protocols, produce plasmids for applications both in vitro and in vivo for various scientific research, flexible scale, up to gram level, high standard quality control, as well as short turn-around time.
According to a senior scientist of Creative Biogene, the plasmid DNA production process includes selection of host strain and growth conditions. high-cell-density fermentation, cell harvesting, lysis and plasmid purification, cell harvesting, lysis and plasmid purification, as well as wide range of quality control testing, such as genomic DNA contamination test, DNA Sequencing, restriction profile analysis and so on.
Plasmid DNAs are extremely vital tools in biotechnology labs. Usually they are used to make many copies of or to express particular genes. Moreover, plasmids are also used to make proteins from the inserted gene. The most obvious advantage is that it is a cheap and easy method to mass produce a gene or the protein. There are plasmid preparation services for many applications, for instance research, preclinical, clinical, and diagnostic applications.
To sum up, the plasmid DNA isolation service will be suitable for transfection and animal studies to both biotechnology researchers and pharmaceutical companies in the near future.
About Creative Biogene
Creative Biogene has been perfecting plasmid DNA production for around ten years. Based on extensive experience in the field, Creative Biogene is helping more and more researchers and developers across 40 countries and regions in the world. With its professional expert team, Creative Biogene will continue to devote itself to the development of life science in the coming years.
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plasmid, gene, DNA