Single-Stranded DNA Binding Protein
Single-stranded specific DNA binding protein.Helix destabilizing protein.Ultrapure recombinant protein.
Reduces formation of secondary DNA structures.Prevents degradation of single stranded DNA by nucleases.Prevents inhibition of PCR by template DNA contaminants.Improves efficiency of DNA amplification by Taq DNA Polymerase.Replaces Hot Start method by assembling PCR reactions.Stabilizes single-stranded regions of DNA for site-specific mutagenesis.Aids completion of restriction enzyme digestion.Improves efficiency of DNA synthesis by T4 DNA Polymerase.Enhances fidelity of modified T4 DNA Polymerase.
20 mM Tris-HCl (pH 7.8 at 22 °C)300 mM NaCl5 mM β-mercaptoethanol0.05% Igepal0.2 mM EDTA50% (v/v) glycerol
Store at -20 °C.