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S1 Nuclease

Cat.No.
EMNT2030
Description
Non-specific endonuclease, active primarily on single-stranded DNA and RNA
Source Description
Aspergillus oryzae
Applications
Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5"-mononucleotides.Cleaves both single-stranded DNA and RNA, with stronger DNAse activity.Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration.Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps.Relaxes/linearizes supercoiled plasmids.Removes protruding single-stranded ends.Used for S1 mapping of nucleic acids.Requires Zn2+ ions for activity.The enzyme is active up to 65 °C.
Reaction Conditions
Incubation at 37 °Cfor 10 minutes in a total reaction volume of 50 μl.
Reaction Buffer
30 mM sodium acetate (pH 4.6).1 mM zinc acetate.50 mM NaCl.0.5 mg/ml activated DNA.5% glycerol.
Size
50000 Units;10000 Units
Storage Buffer
20 mM Tris-HCl (pH 7.5 at 22 °C)50 mM NaCl0.1 mM ZnCl2 50% (v/v) glycerol
Storage Conditions
Store at -20 °C.

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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