RobustCutter Sumo Protease
SUMO Protease, also known as Ulp, is a highly active cysteinyl protease. SUMO Protease cleaves in a highly specific manner and can be used cleave SUMO (Small Ubiquitin-like Modifier) from recombinant fusion proteins. Unlike most proteases, SUMO Protease recognizing the tertiary structure of the ubiquitin-like (UBL) protein instead of an amino acid sequence. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH (pH 7.0–9.0). Following digestion, SUMO Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease.
A recombinant E. coli strain that carries Ulp1 gene fragment from Saccharomyces cerevisiae.
One unit of SUMO Protease cleaves greater than 90% of 100 ug control substrate (SUMO-eGFP) in 1 h at 30°C.
SUMO Protease Storage: 25 mM Tris-HCl (pH 8.0 at 25°C)，1% IGEPAL CA-630 (NP-40), 250 mM NaCl，50 μM DTT，50% (v/v) glycerol.
10× SUMO Protease Buffer (Salt plus):500 mM Tris-HCl (pH 8.0 at 25°C)，2% IGEPAL CA-630 (NP-40)，1.5 M NaCl，10 mM DTT.
10× SUMO Protease Buffer (Salt free):500 mM Tris-HCl (pH 8.0 at 25°C)，2% IGEPAL CA-630 (NP-40)，10 mM DTT.
Store at -20°C