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RecA Protein

Cat.No.
EMOT1128
Description
RecA Protein is a multifunctional DNA-binding protein produced by E.coli that plays an integral role in both homologous recombination and postreplicative DNA repair mechanisms. RecA Protein is commonly used to study the molecular mechanisms involved in homologous recombination.In vitro, RecA Protein helps promote homologous recombination through a multiple-step ATP-dependent process. Initially, the protein binds preferentially to ssDNA forming a nucleoprotein filament. The filament complex binds to naked duplex DNA and searches for regions of homology. Once a region of homology is found, strand displacement and exchange begins.8 Many of the research applications for RecA derive from this recombination process.
Applications
Site-directed mutagenesis through displacement loop structures.1 Targeted site-specific cleavage of small and large DNA.2-4 Enrichment of target sequences from libraries or other DNA pools.5,6 Visualization of DNA for electron microscopy.7 Cloning or other strategies involving use of RecA Protein and a site-specific oligonucleotide to block endonuclease cleavage at the complementary site in a target DNA molecule.
Concentration
5 μg/μl
Size
0.2 mg; 1 mg
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Storage Conditions
store at -20 °C.
Related Products
Cat#
Product Name
Recognition Site
Size
Price
EMOT1107
N/A
500 Units;1000 Units;5000 Units


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Fax: 1-631-614-7828
Email: info@creative-biogene.com

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Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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