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RNase III

Cat.No.
EMNT2026
Description
Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3′ overhangs.5,6 Complete digestion of dsRNA results in dsRNA fragments of 12-15 bp.RNase III can digest dsRNA composed of canonical ribonucleotides as well as DuraScript® RNA produced using Epicentre"s DuraScribe® T7 Transcription Kit, which contains 2′-fluorine-CMP and 2′-fluorine-UMP in place of the canonical CMP and UMP nucleotides.
Applications
Digestion of long dsRNA to short dsRNA. RNA structure studies.1 RNA processing and maturation studies.
Unit Definition
One unit of RNase III, E. coli, solubilizes 1 nmol of ribonucleotide in 30 minutes at 37 °C using poly(A)-poly(U) as the substrate under standard assay conditions.
Concentration
1000 units/ml
Size
50 units
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.5 M NaCl, and 0.1% Triton® X-100.
Related Products
Cat#
Product Name
Recognition Site
Size
Price
EMNT2013
N/A
50 units;200 units


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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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