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Pvu Rts1I Endonuclease

Cat.No.
EMNT2018
Description
digestion conditions, the enzyme selectively cleaves DNA that contains the modified base on one or both strands. The consensus cleavage site of Pvu Rts1I is 5-hmCN11-12/N9-10G.2 Pvu Rts1I serves as a useful tool to analyze 5-hmC patterns within the genome. In addition to distinguishing between 5-mC and 5-hmC in ds DNA, the enzyme can also cleave both glucosylated and nonglucosylated 5-hmC ds DNA.The enzyme is available in a 100-unit size at a concentration of 1 U/μl. The enzyme is supplied with a 10X Reaction Buffer.
Applications
Differentiation of 5-hmC from 5-mC. Cleavage of DNA at glucosylated and nonglucosylated 5-hmC.
Unit Definition
One unit is the amount of Pvu Rts1I needed for complete digestion of 1 μg of T2 gt- DNA in 60 minutes at 30 °C in 1X Reaction Buffer, in a 50-μl reaction.
Concentration
1000 units/ml
Size
100 units
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Storage Conditions
store at -20 °C.
Related Products
Cat#
Product Name
Recognition Site
Size
Price
EMNT2013
N/A
50 units;200 units
EMNT2026
N/A
50 units


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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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