The overexpression of proteins is critical for many applications, including studying the function of the gene, screening activitors or inhibitors of the gene and other assay studies. At Creative Biogene, we have developed a large number of GPCR stable cell lines, Ion channel stable cell lines and Reporter stable cell lines. Besides GPCR, Ion channel and reporter cell lines, scientists at Creative Biogene have also investigated and generated recombinant stable cell lines expressing other popular gene targets such as immune checkpoints, Toll-like receptors (TLRs), Cas9, PDEs, protein kinases etc.
Immune checkpoints are proteins which can turn up or turn down signals in the immune system. Inhibitors of immune-checkpoint proteins have been considered as new targets for cancer immunotherapies. To accelerate research progress of immune-checkpoint proteins, we have developed stable cell lines expressing the most popular immune checkpoints.
Toll-like receptors, abbreviated as TLRs, are a group of proteins that play key roles in innate immune system. TLRs can recognize structurally conserved molecules derived from microbes and further activate immune cell responses. Several TLR stable cell lines have been developed at Creative Biogene for studies of TLRs.
The cyclic nucleotide phosphodiesterases (PDEs) are a family of phosphodiesterases which can hydrolyze nucleoside 3’,5’-cyclic phosphate to nucleoside 5’-phosphate. PDEs play important roles in many cellular signaling pathways by controlling concentrations of cyclic nucleotides. PDE stable cell lines developed by Creative Biogene are useful tools for PDE inhibitor assays.
With the development of CRISPR/Cas9 technology, it is essential to establish Cas9 stable cell lines to increase efficiency of genome editing applications. To meet these needs, we have established Cas9 stable cell lines in some popular host cells.
Protein kinases are a class of kinase enzyme that functionally regulate target proteins by phosphorylation. Phosphorylation are known to regulate many cellular pathways. Kinase mutation or over-expression can lead to cancers or other diseases. Inhibitors of kinases can be used to treat diseases caused by hyperactive protein kinases. Kinase stable cell lines developed at Creative Biogene are ideal for cell-based screening of potential kinase inhibitors.
Table 1. Summary of recombinant stable cell lines with popular genes
(Gene official symbol listed from A to Z, h: human, r: rat, m: mouse, mon: monkey)
|Category||Host Cell||Gene Target|
|Immune Checkpoint & Immune-related Cell Lines||CHO-K1||hBTLA, hC10orf54, hCD160, hCD200R1, hCD27, hCD274, hCD276, hCD38, hCD40, hCD47, hCD80, hCD86, hCTLA4, hHAVCR2, hICOS, hICOSLG, hLAG3, hPDCD1, hPDCD1LG2, hSIRPA, hSIRPG, hSPATA2, hTIGIT, hTNFRSF18, hTNFRSF4, hTNFRSF9, hTNFSF4, hVTCN1, mCd274, mCtla4, mFlt3, mPdcd1 , mTigit, mTnfrsf9, mVtcn1|
|HEK293T||hBTLA, hCD160, hCD226, hCD244, hCD274, hCD276, hCD28, hCD40LG, hCD48, hCD74, hCD80, hCD86, hCEACAM1-L, hCEACAM1-S, hCEACAM5, hCTLA4, hHAVCR1, hHAVCR2, hICOS, hICOSLG, hLAG3, hNT5E, hPDCD1, hPDCD1LG2, hSLAMF1, hTNFRSF17, hTNFRSF4, hTNFSF14, hTNFSF18, hTNFSF8, hVTCN1, mVtcn1, monVTCN1|
|HEK293T-NFkB-Luc||hCD27, hCD40, hTNFRSF14, hTNFRSF8, hTNFRSF9|
|Cas9 Cell Lines||A549, AGS, BXPC-3, H1299, H9 hESC, HEK293T, HeLa, HepG2, HT-29, KATO111, LoVo, MCF-7, MDA-MB-231, Neuro2a, SNU-475|
|TLR Cell Lines||HEK293||hTLR1, hTLR2, hTLR3, hTLR4, hTLR5 , hTLR6, hTLR8, hTLR9, hTLR10, mTLR11, mTLR12|
|PDE Cell Lines||CHO-K1||hPDE9A, hPDE1B|
|HEK293||hPDE2A, hPDE4A, hPDE10A, hPDE1B, hPDE1C|
|Kinase Cell Lines||Ba/F3||hBCR-ABL, hEGFR, hEML4-ALK, hERBB2, hp95HER2, hSLC34A2-ROS1|
• Studying the specific interaction between the target proteins and their ligands
• Screening compounds or drugs
• Research on immune related diseases and finding therapies or exploring drugs
• Creative Biogene provides a variety of stable cell lines with popular gene targets.
• All stable cell lines provided by Creative Biogene are guaranteed free of mycoplasma contamination.
• If you could not find a cell line of interest, our scientists can also tailor one based on your request.