Luc Reporter Cell Line-B16F10 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene and puromycin resistance gene driven by spleen focus-forming virus (SFFV) promoter. It is an ideal positive control for use in bioluminescence assays. In addition, B16F10 cells can form metastases in mouse lungs. The in vivo growth of metastases can be monitored using bioluminescent imaging.