Hep G2 is a perpetual cell line which was derived from the liver tissue of a 15-year-old Caucasian American male with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. Hep G2 cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities. Hep G2 cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress). There is no evidence of a Hepatitis B virus genome in this cell line. The GFP Stable Cell Line-HepG2 constitutively expresses GFP.
Inquiry for instruction of culturing
This cryovial contains at least 1.0 × 10^6 cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The cells are tested free of microbial contamination.