The HCT-116 line was isolated from a male with colonic carcinoma. The cells are positive for keratin by immunoperoxidase staining and are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.The GFP Stable Cell Line-HCT-116 constitutively expresses GFP.
Inquiry for instruction of culturing
This cryovial contains at least 1.0 × 10^6 cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The cells are tested free of microbial contamination.