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E. coli Fpg

Cat.No.
EMOT1108
 
Description
Fpg (also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, and 8-oxoguanine DNA glycosylase) participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3" and 5" phosphodiester bonds at the AP site, producing a 1 base gap in the DNA and 3" and 5" phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy- guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1- fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
 
Source Description
A recombinant E. coli strain carrying the cloned fpg gene.
 
Unit Definition
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34mer oligo-nucleotide duplex containing an 8-oxoguanine base in 1 hour at 37 °C in 1X Yellow Reaction Buffer.
 
Reaction Buffer
100 mM BisTris-Propane-HCl100 mM MgCl210 mM DTTpH 7.0 @ 25 °C
 
Concentration
8000 U/ml
 
Size
4000 Units
 
Storage Buffer
20 mM Tris-HCl50 mM NaCl1.0 mM DTT0.1 mM EDTA 50% glycerolpH 8.0 at 25 °C
 
Storage Conditions
storge at -25 to -15 °C.
 

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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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